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KMID : 1025520050470020167
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Journal of Animal Science and Technology
2005 Volume.47 No. 2 p.167 ~ p.176
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Characterization of Phosphatidylinositol Glycan, Class K (PIGK) Gene and Analysis of Association with Quantitative Traits in Pigs
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Lim Hyun-Tae
Kim J.-H.
Choi Bong-Hwan
Lee Sang-Ho
Park Eung-Woo
Kim Tae-Hun
Cho In-Cheol
Oh S.-J.
Lee Jung-Gyu
Jeon Jin-Tae
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Abstract
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PIGK(phosphatidylinositol glycan, class K) is a subunit of GPI transamidase that cleaves the signal peptide in proproteins and replaces it with GPI. In addition, the structure and synthesis of GPI are critically involved in some of the cellular actions of insulin. Therefore, PIGK would be essential for mammalian development and many specific cellular functions as well as for metabolic activity of insulin associated with GPI. Two types of full-length cDNAs of porcine PIGK were cloned through RT-PCR and RACE experiments. One is thought to be a normal form(consist of 395 amino acids) and the other is considered as an alternative spliced form(consist of 371 amino acids) which contains additional 63 bps in intron 7. Since a stop codon was contained within the insertion, the spliced form has a shorter coding sequence than that of normal form. A missense mutation (T314I) in exon 6 was detected and used for genotyping to estimate association with the growth and fat deposition traits for 545 F2 animals(Korean native boars×Landrace). From the PCR-RFLP analysis using HpyCH4III, CT genotype showed highly significant relationship(P<0.01) with carcass fat contents.
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KEYWORD
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PIGK, Missense mutation, Association, Fat deposition traits
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